Molecular Laboratory Testing Services
Helix Biological Laboratory has become an innovator in applying molecular testing technologies toward a variety of genetic studies of plant, animal, and microbial organisms found in terrestrial, freshwater, and marine environments. We offer laboratory testing services to the general public, academics, university affiliates, researchers, law enforcement agencies, attorneys, and other laboratories.
What is Molecular Lab Testing?
Molecular laboratory testing is a broad term that refers to the detection, identification, and quantification of specific DNA or RNA sequences in an organism or sample. Samples tested vary widely and include organic animal tissues like skin tissue and blood, organic materials including soil and plant matter, bacterial and fungal residues, and environmental samples such as water.
PCR and DNA Sequencing
Molecular lab testing provides a high level of accuracy and reliability using “tools” that include nucleic acid amplification tests (NAAT) such as the Polymerase Chain Reaction (PCR) technique and DNA sequencing. PCR and DNA sequencing have the ability to exploit unique, signature sequences (referred to as DNA markers) in the genomic DNA of organisms.
PCR is an in vitro chemical reaction that can exponentially “amplify” the targeted nucleic acid to detectable levels. Amplification of a DNA marker requires the design of small strands of single-stranded DNA called “primers,” which precisely match a specific and unique area of the organism’s genome and flank the target DNA marker. During a PCR assay, the primers attach or “anneal” to the specific areas of the genome of the organism and provide the basis for amplification of the target DNA marker.
The genomic region where the primers attach or anneal is amplified in repeated cycles. These cycles very closely resemble the natural DNA replication that occurs in biological cells. One cycle of PCR doubles the amount of genetic material present in the starting sample. Performing multiple cycles of PCR produces millions to billions of copies of the target DNA marker. Additionally, a molecular PCR test can detect the target DNA marker even if it is only present in small amounts in the sample.
When coupled with PCR and DNA sequencing, target DNA markers give molecular testing the ability to detect and identify both macro organisms and microorganisms, perform complex metagenomic analysis, locate unique, signature DNA sequences within genes, and much more.
Molecular Testing For DNA Sequence Based Studies
Through the experience and expertise of our laboratory staff, we provide services that include the extraction of DNA from biological samples, and PCR amplification and DNA sequencing of specific nuclear and mitochondrial marker genes that generate data that are used for the following:
- Population Genetic Studies
- Metagenomic Studies
- Evolutionary Biology Studies
- Molecular Phylogenetic Reconstructions
- Species Identification
- Ecological Genetic Studies
- Environmental (eDNA) Analysis
- Genetically Modified Organism (GMO) Analysis
Experienced Assay Design Services
Our scientists are experienced with oligonucleotide primer design, editing and aligning raw DNA sequences, phylogenetic reconstruction, and molecular data analysis. Helix Biological Laboratory does not automate any portion of our analytical methods.
Partner with Helix Biological Laboratory
Highly complex molecular testing is performed in a laboratory that is specially outfitted with the equipment and resources necessary to carry out such highly technical work and is performed by trained laboratory professionals with a high level of expertise.
Our specialist scientists at Helix Biological Laboratory have extensive expertise in creating and performing molecular assays for our clients. Contact us today to consult with our scientific team about your upcoming project.